![]() The explanation in the workshop is not in a sequence just jumping within different examples I think it is hard for the beginner to understand how to start analyze certain case if follow such video. Im confused at first I start with threshold and I got bad results then I tried to do segmentation and I cant got the same image that you have attached to me. Im trying to follow the steps in the workshop as you recommended but its not worth to me. ![]() If you are just getting started, we recommend downloading/using Fiji. * NOTE: Fiji is Just ImageJ - it is simply a distribution of ImageJ that comes with a bunch of plugins bundled - ready for you to use out-of-the-box. I hope this helps get you started! If you have more questions - just ask. ![]() “Segmentation in Fiji” workshop and corresponding slides.Principles page - collection of principles for the entire image analysis process, from acquisition to processing to analysis.“Introduction to Fiji” workshop and corresponding slides- worth the time to get a solid intro.ImageJ wiki - the best place to learn everything about ImageJ/Fiji!!.Here are some other helpful tips for Segmentation in ImageJ/Fiji: Trainable Weka Segmentation (TWS) plugin and it is scriptable via macro code - a great tool for segmentation that comes directly with Fiji*. For your particular images… I’d point you to either:Ĭolor Threshold tool… just be sure to apply the same settings to all images (assuming you acquired them with the same exact settings all on the same system…). Sure you can use ImageJ/Fiji for this… what you need to do is Segment those regions to then measure their areas.
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